In a groundbreaking development that could revolutionize clinical diagnostics and energy sector applications, researchers have unveiled a novel biosensor for uric acid (UA) detection. The innovation, spearheaded by Samira Saidi from the Department of Chemistry at the University of Shahid Bahonar Kerman in Iran, integrates bimetallic Fe–Ni-MOF nanoparticles with reduced graphene oxide (RGO) nanosheets and immobilized uricase on a glassy carbon electrode (GCE). This cutting-edge design promises to enhance the accuracy and efficiency of UA detection, with significant implications for healthcare and beyond.
The biosensor’s unique architecture leverages the synergistic electrocatalytic activity of Fe–Ni bimetallic centers and the high conductivity of RGO. “The combination of these materials not only improves the sensor’s performance but also ensures a statistically validated model through systematic optimization,” explains Saidi. The team employed central composite design (CCD) and response surface methodology to fine-tune critical experimental parameters, including Fe–Ni-MOF and RGO concentrations, enzyme volume, and pulse potential.
Under optimal conditions, the biosensor demonstrated a sharp anodic response at 0.31V in phosphate buffer (pH 7.4). Its broad linear range (0.005–5000 μM) and ultralow detection limit of 0.21 μM set a new benchmark in UA sensing technology. “This performance outperforms most state-of-the-art UA sensors, making it a game-changer in the field,” Saidi asserts.
The practical applications of this innovation are vast. In the healthcare sector, the biosensor’s accuracy and reproducibility make it ideal for clinical diagnostics, particularly in monitoring conditions like gout and kidney disorders. Its ability to achieve excellent recoveries (99.1–101.7%) in real human plasma and urine samples underscores its reliability.
Beyond healthcare, the energy sector stands to benefit significantly. Uric acid sensing can play a crucial role in biofuel production and environmental monitoring. The biosensor’s high sensitivity and selectivity can aid in the detection of UA in various industrial processes, ensuring quality control and environmental safety.
The research, published in the Journal of Experimental Nanoscience (translated to English as “Experimental Nanoscience Journal”), represents a significant leap forward in biosensor technology. As the world continues to seek innovative solutions for healthcare and energy challenges, this development offers a promising path forward.
The implications of this research extend beyond immediate applications. It paves the way for future advancements in biosensor technology, encouraging further exploration of synergistic materials and optimized enzyme loading. As Saidi notes, “This is just the beginning. The potential for further innovation in this field is immense.”
In conclusion, the novel Fe–Ni-MOF/RGO nanosheet-based uricase biosensor on a glassy carbon electrode marks a pivotal moment in the evolution of UA detection. Its superior performance and broad applicability position it as a key player in both clinical diagnostics and industrial applications, shaping the future of healthcare and energy sectors.